Dr. Martin Gilar | Chromatography | Best Researcher Award
Waters Corporation, United States
👨🎓Profiles
🎓 Early Academic Pursuits
Dr. Martin Gilar completed his Ph.D. in Analytical Chemistry in 1996 from the Institute of Chemical Technology in Prague. His thesis, focused on the chiral separation of dihydropyridine calcium antagonists, established his early interest in complex analytical challenges. With an MSc in Chemistry from the same institution, Dr. Gilar also gained expertise in organic technology, further refining his skills with a certificate in Pharmaceutical Analytical Methods from the Institute of Medical Postgraduate Education, Charles University.
💼 Professional Endeavors
He began his career as a Senior Staff Scientist at the Faculty Hospital of Charles University, Prague, where he led clinical studies and developed methods for drug monitoring and enantioseparation. His subsequent work at the National Institute of Health in Prague saw him pioneering capillary gas chromatography and high-performance liquid chromatography methods to phenotype cytochrome P450 markers. He joined Waters Corporation in Milford, Massachusetts, where he continues to serve as a Research Fellow. Over more than two decades at Waters, he has made transformative advancements, from LC-MS analysis of oligonucleotides to multi-dimensional separations of complex proteins and peptides. He also contributed to the commercialization of impactful products like RapiGest and HILIC SPE plates, underscoring his contributions to both academia and industry.
🔬 Contributions and Research Focus
His research is deeply rooted in separation science, particularly in liquid chromatography (LC), ultra-performance liquid chromatography (UPLC), and LC-MS methods. He has explored diverse biomolecules, from oligonucleotides to glycopeptides, establishing techniques that have refined the precision of these separations. His pioneering work includes the development of multi-dimensional protein separation techniques with MS detection and contributions to capillary electrophoresis instrumentation.
🌍 Impact and Influence
With over 80 publications in peer-reviewed journals, Dr. Gilar has made a significant impact on analytical chemistry, particularly in chromatography and oligonucleotide analysis. His methodologies are widely adopted in fields spanning pharmaceutical sciences, biotechnology, and clinical diagnostics. His expertise is further recognized by prestigious accolades such as the Jaroslav Janak Medal from the Czech Academy of Science and the Chromatographic Society Jubilee Medal, underscoring his lasting influence in chromatography.
📈 Academic Citations and Patents
His innovations are not only influential in publications but also in patents. He holds several U.S. and international patents, including patents for solid-phase extraction devices and mass-spectrometric purification methods for biopolymers. These innovations have facilitated efficient sample preparation and complex biopolymer purification, enabling more accurate and high-throughput analyses. His work continues to be highly cited in academic circles, reflecting its foundational impact on modern analytical techniques.
🛠️ Technical Skills
His technical expertise spans LC, UPLC, HPLC, and capillary electrophoresis for both qualitative and quantitative analysis. He has a deep knowledge of ion-pairing techniques, hydrophilic interaction chromatography (HILIC), and two-dimensional liquid chromatography (2D-LC). His extensive experience with mass spectrometry (MS) and advanced detection systems has also supported his development of industry-leading techniques in protein, peptide, and oligonucleotide analysis.
🧑🏫 Teaching Experience and Mentorship
Throughout his career, Dr. Gilar has been committed to teaching and mentoring the next generation of scientists. His early role as a Staff Scientist at Northeastern University included supervising Ph.D. students and developing analytical instrumentation. His ongoing work at Waters Corporation has also involved leading and managing research teams, fostering a collaborative environment that drives innovative research.
🌟 Legacy and Future Contributions
His contributions have established a legacy in chromatography, particularly in innovative separation techniques and practical applications in biotechnology. His continued research at Waters Corporation promises to advance analytical chemistry, driving the field toward more precise, efficient, and reliable methods. As he remains active in both research and mentorship, His influence is set to endure, shaping the analytical landscape for years to come.
📖Notable Publications
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"Impact of mobile and stationary phases on siRNA duplex stability in liquid chromatography"
- Authors: Gilar, M., Redstone, S., Gomes, A.
- Journal: Journal of Chromatography A
- Year: 2024
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"Impact of ion-pairing systems choice on diastereomeric selectivity of phosphorothioated oligonucleotides in reversed-phase liquid chromatography"
- Authors: Vosáhlová, Z., Gilar, M., Kalíková, K.
- Journal: Journal of Chromatography A
- Year: 2024
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"Hydrophilic interaction liquid chromatography with mass spectrometry for the separation and identification of antisense oligonucleotides impurities and nusinersen metabolites"
- Authors: Vosáhlová, Z., Kalíková, K., Gilar, M., Mazurkiewicz-Bełdzińska, M., Studzińska, S.
- Journal: Journal of Chromatography A
- Year: 2024
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"Ion-pair reversed-phase and hydrophilic interaction chromatography methods for analysis of phosphorothioate oligonucleotides"
- Authors: Gilar, M., Koshel, B.M., Birdsall, R.E.
- Journal: Journal of Chromatography A
- Year: 2023
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"Liquid Chromatography Methods for Analysis of mRNA Poly(A) Tail Length and Heterogeneity"
- Authors: Gilar, M., Doneanu, C., Gaye, M.M.
- Journal: Analytical Chemistry
- Year: 2023
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"Mixed-mode column allows simple direct coupling with immobilized enzymatic reactor for on-line protein digestion"
- Authors: Vosáhlová-Kadlecová, Z., Gilar, M., Molnárová, K., Kozlík, P., Kalíková, K.
- Journal: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
- Year: 2023